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UniverSC Version 1.1.0

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@TomKellyGenetics TomKellyGenetics released this 12 May 01:44
· 243 commits to master since this release

12 May, 2021

  • add support for new technologies:

    • 5′ scRNA technologies (e.g., Smart-Seq3, ICELL8 5′ , STRT-Seq)
    • full support for combinatorial indexing (e.g., BD Rhapsody, Microwell-Seq, SCI-RNA-Seq, SPLiT-Seq, SureCell/ddSEQ)
    • full support for dual indexes (e.g., Fluidigm C1, ICELL8 full-length, inDrops v3, SCI-RNA-Seq, Smart-Seq3)
    • non-UMI technologies (e.g., ICELL8-v2, Quartz-Seq, RamDA-Seq, Smart-Seq2, STRT-Seq)
  • add support for barcodes longer than 16 bp

  • compatilibility with counting reads for non-UMI technologies

    • generates a mock UMI for non-UMI technologies
    • implements an option to replace UMI with mock UMI to force counting reads only
  • support custom whitelists from mixed samples with a low fraction of reads

  • update test cases for renaming files and compressed inputs

  • bug fixes for computing summary statistics

  • addresses peer-review comments