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RPW
Sadjad F Baygi edited this page Jan 22, 2023
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A threshold for RPW is helpful to remove false positive rectangular-shape peaks which are over-smoothed and pass other peak quality metrics. It is advised to use mixed alternative tightened and relaxed criteria for RPW and baseline S/N since both parameters represent the geometry of the peaks. The recommended range for RPW is <= 0.8 when baseline S/N threshold is not used for data reduction.
For instance, this peak can be skipped with an RPW threshold. This is an example, where the baseline S/N threshold was slightly above the limit (>= 2) and this peak was detected when no RPW threshold was applied.
![](https://github.com/idslme/IDSL.IPA/raw/main/IPA_educational_files/RPW/IPA_EIC_003.mzML_5098_MZ_238.96808_RT_6.671_.png?raw=true)
This peak is an example of RPW measurement for a true positive peak.
![](https://github.com/idslme/IDSL.IPA/raw/main/IPA_educational_files/RPW/IPA_EIC_003.mzML_16604_MZ_617.32535_RT_5.513_.png?raw=true)