STM32F407 Programer for the STMPRO ECU.
firmware.bin file is Firmware 202108.

Only available for windows OS currently.
Zadig-2.5.exe needs to be installed first
\
Plug the ecu into USB\
In Tunerstudio end tab "Hardware testing"
STM32 Commands
You will see a button 
"Reboot to bootloader"

Normally with the ecu connected Tuner studio should go grey and stay that way, if it reconnects it didn't work and you have to try again.

If this doesn't work for you, you can use the manual mode, Using a jumper as shown in the "Manual DFU mode" image.

windows should say something about a stm32 Bootloader. when this happens you need to run Zadig.
View the image in the download to see the settings needed.
Run Zadig
goto options on the top and select list all devices.
then from the dropdown menu select STM32Bootloader.
If you see "Black_f407VE in CDC" its not in DFU mode.
there are to boxes with a arrow inbetween on the right hand side box scroll through untill you see
"Winusb(v6.x.xxxx)"
click reinstall driver
It should new be ready to program.
run Uploadfirmware.bat and it should open up a dos screen 
identify the cpu and start programing.
once programed unplug ecu and reconnect to TS, it will ask to update the ini file
the new ini file is Speeduino.ini in this download directory.