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I used ONT DRS RNA004 data, I don't know how to run IsoAnnot #2

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Tang-pro opened this issue Feb 11, 2025 · 0 comments
Open

I used ONT DRS RNA004 data, I don't know how to run IsoAnnot #2

Tang-pro opened this issue Feb 11, 2025 · 0 comments

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@Tang-pro
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Tang-pro commented Feb 11, 2025

Hi @aarzalluz @carolinamonzo

I am using ONT DRS, RNA004 kit, I don't know how to run IsoAnnot and also SQANTI3 comes with this IsoAnnot;
And my senior brother gave me the simple GTF file, do I need to use this GTF as a reference input, or run it directly without gtf

./isoannot.sh --database mytranscripts --species jh1 --config fasta_cdna=/public/home/JH1transcripts.fa

the error is The snakefile or configfile requested do not exist. Please, make sure that you are using a supported species.

And my species is cotton, a non-model species, how to write config.yaml

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