oPool⁺ display: A high-throughput cell-free screening platform for natively paired antibodies

Welcome! This README describe the workflow in manuscript: Highthroughput synthesis and specificity characterization of natively paired antibodies using oPool⁺ display.

Contents

• Introduction
• Environment setup
• oPool⁺ display design
• oPool⁺ display results

Introduction

oPool⁺ display combines oligo pool synthesis and mRNA display to construct and characterize the specificity of many natively paired antibodies in parallel. As a proof-of-concept, we applied oPool⁺ display to rapidly screen the binding activity of >300 previously uncharacterized influenza hemagglutinin (HA) antibodies against 9 HA variants via 16 different screens. Over 5,000 binding tests were performed in 3-5 days. This repository, therefore, contains two parts: 1. the oligo sequence desgins for library assmebly and 2. the screening result and analyses.

Environment setup

To create the same working environment:

conda env create -f environment.yml

Oligo pool seqeunce design

Please proceed to the oPool_design folder

Screening and experimental data analyses

Please proceed to the oPool_display folder