Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in metazoans with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then "binned" together based on genomic location. Each bin is then annotated with databases of mature miRNAs, hairpins, and other types of RNAs (the databases may be derived from miRBase, Ensembl's BioMart, other databases, or may be custom built by the user).

Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in metazoans with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then "binned" together based on genomic location. Each bin is then annotated with databases of mature miRNAs, hairpins, and other types of RNAs (the databases may be derived from miRBase, Ensembl's BioMart, other databases, or may be custom built by the user).

Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in metazoans with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then "binned" together based on genomic location. Each bin is then annotated with databases of mature miRNAs, hairpins, and other types of RNAs (the databases may be derived from miRBase, Ensembl's BioMart, other databases, or may be custom built by the user).

Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in metazoans with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then "binned" together based on genomic location. Each bin is then annotated with databases of mature miRNAs, hairpins, and other types of RNAs (the databases may be derived from miRBase, Ensembl's BioMart, other databases, or may be custom built by the user).

Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in metazoans with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then "binned" together based on genomic location. Each bin is then annotated with databases of mature miRNAs, hairpins, and other types of RNAs (the databases may be derived from miRBase, Ensembl's BioMart, other databases, or may be custom built by the user).

Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in metazoans with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then "binned" together based on genomic location. Each bin is then annotated with databases of mature miRNAs, hairpins, and other types of RNAs (the databases may be derived from miRBase, Ensembl's BioMart, other databases, or may be custom built by the user).

Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in metazoans with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then "binned" together based on genomic location. Each bin is then annotated with databases of mature miRNAs, hairpins, and other types of RNAs (the databases may be derived from miRBase, Ensembl's BioMart, other databases, or may be custom built by the user).

Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in chordates and vertebrates with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then “binned” together based on genomic location. Each bin is then annotated with miRBase mature sequences and hairpins, as well as other types of RNA obtained from Ensembl’s Biomart.

Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis

Prost! (PRocessing Of Small Transcripts) is a python application that quantifies and annotates microRNA (miRNA) expression in chordates and vertebrates with assembled genomes. Prost! works by counting short transcripts within a user-specifiable length range. These counted transcripts are aligned to a user specifiable genome allowing for post-transcriptional modification (e.g. untemplated additions, editing, alternative cutting) and then “binned” together based on genomic location. Each bin is then annotated with miRBase mature sequences and hairpins, as well as other types of RNA obtained from Ensembl’s Biomart.

Public Release of Prost

This is a release of Prost! to be tagged with a DOI by Zenodo, so that Prost! is citable.