diff --git a/test/tarantool-tests/gh-6227-bytecode-allocator-for-comparisons.test.lua b/test/tarantool-tests/gh-6227-bytecode-allocator-for-comparisons.test.lua
new file mode 100644
index 0000000000..9788923ad7
--- /dev/null
+++ b/test/tarantool-tests/gh-6227-bytecode-allocator-for-comparisons.test.lua
@@ -0,0 +1,54 @@
+local tap = require('tap')
+local test = tap.test('gh-6227-bytecode-allocator-for-comparisons')
+test:plan(1)
+
+-- Test file to demonstrate assertion failure during recording
+-- wrong allocated bytecode for comparisons.
+-- See also https://github.com/tarantool/tarantool/issues/6227.
+
+-- Need function with RET0 bytecode to avoid reset of
+-- the first JIT slot with frame info. Also need no assignments
+-- by the caller.
+local function empty() end
+
+local uv = 0
+
+-- This function needs to reset register enumerating.
+-- `J->maxslot` is initialized with `nargs` (i.e. zero in this
+-- case) in `rec_call_setup()`.
+local function bump_frame()
+  -- First call function with RET0 to set TREF_FRAME in the
+  -- last slot.
+  empty()
+  -- The old bytecode to be recorded looks like the following:
+  -- 0000  . FUNCF    4
+  -- 0001  . UGET     0   0      ; empty
+  -- 0002  . CALL     0   1   1
+  -- 0000  . . JFUNCF   1   1
+  -- 0001  . . RET0     0   1
+  -- 0002  . CALL     0   1   1
+  -- 0003  . UGET     0   0      ; empty
+  -- 0004  . UGET     3   1      ; uv
+  -- 0005  . KSHORT   2   1
+  -- 0006  . ISLT     3   2
+  -- Test ISGE or ISGT bytecode. These bytecodes swap their
+  -- operands (consider ISLT above).
+  -- Two calls of `empty()` function in a row is necessary for 2
+  -- slot gap in LJ_FR2 mode.
+  -- Upvalue loads before KSHORT, so the difference between slot
+  -- for upvalue `empty` (function to be called) and slot for
+  -- upvalue `uv` is more than 2. Hence, TREF_FRAME slot is not
+  -- rewritten by the bytecode after return from `empty()`
+  -- function as expected. That leads to recording slots
+  -- inconsistency and assertion failure at `rec_check_slots()`.
+  empty(1>uv)
+end
+
+jit.opt.start('hotloop=1')
+
+for _ = 1, 3 do
+  bump_frame()
+end
+
+test:ok(true)
+os.exit(test:check() and 0 or 1)