The original wetlab protocol for OCTOPUS can be found on our Bench Protocol page.
We developed a protocol that uses RCA (rolling circle amplification) to avoid the time consuming and error-prone lysate generation step. We highly recommend this updated Bench Protocol with RCA, which you can read about here.
You can refer to these resources for information on our computational protocol:
For a deeper dive into the implementation details of OCTOPUS, you can refer to these pages: