diff --git a/pkg/karmadactl/cmdinit/cert/cert.go b/pkg/karmadactl/cmdinit/cert/cert.go
index 41c66dab9a64..50b38e48f8d8 100644
--- a/pkg/karmadactl/cmdinit/cert/cert.go
+++ b/pkg/karmadactl/cmdinit/cert/cert.go
@@ -22,6 +22,7 @@ import (
 	"crypto/elliptic"
 	"crypto/rand"
 	"crypto/rsa"
+	"crypto/tls"
 	"crypto/x509"
 	"crypto/x509/pkix"
 	"encoding/pem"
@@ -261,11 +262,12 @@ func NewCertConfig(cn string, org []string, altNames certutil.AltNames, notAfter
 }
 
 // GenCerts Create CA certificate and sign etcd karmada certificate.
-func GenCerts(pkiPath string, etcdServerCertCfg, etcdClientCertCfg, karmadaCertCfg, apiserverCertCfg, frontProxyClientCertCfg *CertsConfig) error {
-	caCert, caKey, err := NewCACertAndKey("karmada")
+func GenCerts(pkiPath, caCertPath, caKeyPath string, etcdServerCertCfg, etcdClientCertCfg, karmadaCertCfg, apiserverCertCfg, frontProxyClientCertCfg *CertsConfig) error {
+	caCert, caKey, err := getCertAndKey(caCertPath, caKeyPath)
 	if err != nil {
 		return err
 	}
+
 	if err = WriteCertAndKey(pkiPath, globaloptions.CaCertAndKeyName, caCert, caKey); err != nil {
 		return err
 	}
@@ -309,6 +311,27 @@ func GenCerts(pkiPath string, etcdServerCertCfg, etcdClientCertCfg, karmadaCertC
 	return genEtcdCerts(pkiPath, etcdServerCertCfg, etcdClientCertCfg)
 }
 
+func getCertAndKey(caCertPath, caKeyPath string) (caCert *x509.Certificate, caKey *crypto.Signer, err error) {
+	if caKeyPath != "" && caCertPath != "" {
+		certificate, err := tls.LoadX509KeyPair(caCertPath, caKeyPath)
+		if err != nil {
+			return nil, nil, err
+		}
+		caCert, err = x509.ParseCertificate(certificate.Certificate[0])
+		if err != nil {
+			return nil, nil, err
+		}
+		key := certificate.PrivateKey.(crypto.Signer)
+		caKey = &key
+	} else {
+		caCert, caKey, err = NewCACertAndKey("karmada")
+		if err != nil {
+			return nil, nil, err
+		}
+	}
+	return caCert, caKey, nil
+}
+
 func genEtcdCerts(pkiPath string, etcdServerCertCfg, etcdClientCertCfg *CertsConfig) error {
 	etcdCaCert, etcdCaKey, err := NewCACertAndKey("etcd-ca")
 	if err != nil {
diff --git a/pkg/karmadactl/cmdinit/cert/cert_test.go b/pkg/karmadactl/cmdinit/cert/cert_test.go
index 750573b92770..e069630dabee 100644
--- a/pkg/karmadactl/cmdinit/cert/cert_test.go
+++ b/pkg/karmadactl/cmdinit/cert/cert_test.go
@@ -20,6 +20,7 @@ import (
 	"fmt"
 	"net"
 	"os"
+	"path/filepath"
 	"testing"
 	"time"
 
@@ -29,11 +30,33 @@ import (
 )
 
 const (
-	TestCertsTmp = "./test-certs-tmp"
+	TestCertsTmp   = "./test-certs-tmp"
+	TestCaCertPath = "./test-ca.crt"
+	TestCaKeyPath  = "./test-ca.key"
 )
 
+func writeMockCert() {
+	certData := []byte("-----BEGIN CERTIFICATE-----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\nSmz0jaVWUjrO2GsIrw==\n-----END CERTIFICATE-----")
+	if err := os.MkdirAll(filepath.Dir(TestCaCertPath), os.FileMode(0755)); err != nil {
+		fmt.Println(err)
+	}
+	if err := os.WriteFile(TestCaCertPath, certData, os.FileMode(0644)); err != nil {
+		fmt.Println(err)
+	}
+	keyData := []byte("-----BEGIN EC PRIVATE KEY-----\nMHcCAQEEIIPimXOHhF5n74QbTprxcvLE/WURZL3w4IuhY6B/LfufoAoGCCqGSM49\nAwEHoUQDQgAEvPbgHojXKYeQPxVg1tibsnU+Q9EqXAg/HUbMWb3YTIrmeQ1jiYyY\ngPBOhwozTwgE0EGd6y98fqKkGpVT3kiiEA==\n-----END EC PRIVATE KEY-----")
+	if err := os.MkdirAll(filepath.Dir(TestCaKeyPath), os.FileMode(0755)); err != nil {
+		fmt.Println(err)
+	}
+	if err := os.WriteFile(TestCaKeyPath, keyData, os.FileMode(0644)); err != nil {
+		fmt.Println(err)
+	}
+}
+
 func TestGenCerts(_ *testing.T) {
 	defer os.RemoveAll(TestCertsTmp)
+	defer os.RemoveAll(TestCaCertPath)
+	defer os.RemoveAll(TestCaKeyPath)
+	writeMockCert()
 
 	notAfter := time.Now().Add(Duration365d * 10).UTC()
 	namespace := "kube-karmada"
@@ -101,7 +124,7 @@ func TestGenCerts(_ *testing.T) {
 	apiserverCertCfg := NewCertConfig("karmada-apiserver", []string{""}, karmadaAltNames, &notAfter)
 	frontProxyClientCertCfg := NewCertConfig("front-proxy-client", []string{}, certutil.AltNames{}, &notAfter)
 
-	if err := GenCerts(TestCertsTmp, etcdServerCertConfig, etcdClientCertCfg, karmadaCertCfg, apiserverCertCfg, frontProxyClientCertCfg); err != nil {
+	if err := GenCerts(TestCertsTmp, TestCaCertPath, TestCaKeyPath, etcdServerCertConfig, etcdClientCertCfg, karmadaCertCfg, apiserverCertCfg, frontProxyClientCertCfg); err != nil {
 		fmt.Println(err)
 	}
 }
diff --git a/pkg/karmadactl/cmdinit/cmdinit.go b/pkg/karmadactl/cmdinit/cmdinit.go
index ab946f4ec365..9ace6431a74d 100644
--- a/pkg/karmadactl/cmdinit/cmdinit.go
+++ b/pkg/karmadactl/cmdinit/cmdinit.go
@@ -125,6 +125,8 @@ func NewCmdInit(parentCommand string) *cobra.Command {
 	flags.StringVar(&opts.ExternalIP, "cert-external-ip", "", "the external IP of Karmada certificate (e.g 192.168.1.2,172.16.1.2)")
 	flags.StringVar(&opts.ExternalDNS, "cert-external-dns", "", "the external DNS of Karmada certificate (e.g localhost,localhost.com)")
 	flags.DurationVar(&opts.CertValidity, "cert-validity-period", cert.Duration365d, "the validity period of Karmada certificate (e.g 8760h0m0s, that is 365 days)")
+	flags.StringVarP(&opts.CaCertPath, "ca-cert-path", "", "", "The path of CA cert, if not set, will gen new, the option must be specified together with ca-key-path")
+	flags.StringVarP(&opts.CaKeyPath, "ca-key-path", "", "", "The path of CA key, if not set, will gen new,  the option must be specified together with ca-cert-path")
 	// Kubernetes
 	flags.StringVarP(&opts.Namespace, "namespace", "n", "karmada-system", "Kubernetes namespace")
 	flags.StringVar(&opts.StorageClassesName, "storage-classes-name", "", "Kubernetes StorageClasses Name")
diff --git a/pkg/karmadactl/cmdinit/kubernetes/deploy.go b/pkg/karmadactl/cmdinit/kubernetes/deploy.go
index 28d7262ee8d4..a5b437f638aa 100644
--- a/pkg/karmadactl/cmdinit/kubernetes/deploy.go
+++ b/pkg/karmadactl/cmdinit/kubernetes/deploy.go
@@ -171,6 +171,8 @@ type CommandInitOption struct {
 	KarmadaAPIServerIP                 []net.IP
 	HostClusterDomain                  string
 	WaitComponentReadyTimeout          int
+	CaCertPath                         string
+	CaKeyPath                          string
 }
 
 func (i *CommandInitOption) validateLocalEtcd(parentCommand string) error {
@@ -353,7 +355,7 @@ func (i *CommandInitOption) genCerts() error {
 	apiserverCertCfg := cert.NewCertConfig("karmada-apiserver", []string{""}, karmadaAltNames, &notAfter)
 
 	frontProxyClientCertCfg := cert.NewCertConfig("front-proxy-client", []string{}, certutil.AltNames{}, &notAfter)
-	if err = cert.GenCerts(i.KarmadaPkiPath, etcdServerCertConfig, etcdClientCertCfg, karmadaCertCfg, apiserverCertCfg, frontProxyClientCertCfg); err != nil {
+	if err = cert.GenCerts(i.KarmadaPkiPath, i.CaCertPath, i.CaKeyPath, etcdServerCertConfig, etcdClientCertCfg, karmadaCertCfg, apiserverCertCfg, frontProxyClientCertCfg); err != nil {
 		return err
 	}
 	return nil