diff --git a/pkg/dependenciesdistributor/dependencies_distributor.go b/pkg/dependenciesdistributor/dependencies_distributor.go
index bb3e1b17fe22..dcd1a747e0ed 100644
--- a/pkg/dependenciesdistributor/dependencies_distributor.go
+++ b/pkg/dependenciesdistributor/dependencies_distributor.go
@@ -560,6 +560,18 @@ func (d *DependenciesDistributor) createOrUpdateAttachedBinding(attachedBinding
 	bindingKey := client.ObjectKeyFromObject(attachedBinding)
 	err := d.Client.Get(context.TODO(), bindingKey, existBinding)
 	if err == nil {
+		// If this binding exists and its owner is not the input object, return error and let garbage collector
+		// delete this binding and try again later. See https://github.com/karmada-io/karmada/issues/6034.
+		if ownerRef := metav1.GetControllerOfNoCopy(existBinding); ownerRef != nil && ownerRef.UID != attachedBinding.OwnerReferences[0].UID {
+			return fmt.Errorf("failed to update resourceBinding(%s) due to different owner reference UID, will "+
+				"try again later after binding is garbage collected, see https://github.com/karmada-io/karmada/issues/6034", bindingKey)
+		}
+
+		// If the spec.Placement is nil, this means that existBinding is generated by the dependency mechanism.
+		// If the spec.Placement is not nil, then it must be generated by PropagationPolicy.
+		if existBinding.Spec.Placement == nil {
+			existBinding.Spec.ConflictResolution = attachedBinding.Spec.ConflictResolution
+		}
 		existBinding.Spec.RequiredBy = mergeBindingSnapshot(existBinding.Spec.RequiredBy, attachedBinding.Spec.RequiredBy)
 		existBinding.Labels = util.DedupeAndMergeLabels(existBinding.Labels, attachedBinding.Labels)
 		existBinding.Spec.Resource = attachedBinding.Spec.Resource
diff --git a/pkg/dependenciesdistributor/dependencies_distributor_test.go b/pkg/dependenciesdistributor/dependencies_distributor_test.go
index c1419835c624..31443d69bd60 100644
--- a/pkg/dependenciesdistributor/dependencies_distributor_test.go
+++ b/pkg/dependenciesdistributor/dependencies_distributor_test.go
@@ -34,11 +34,13 @@ import (
 	"k8s.io/client-go/dynamic"
 	dynamicfake "k8s.io/client-go/dynamic/fake"
 	"k8s.io/client-go/kubernetes/scheme"
+	"k8s.io/utils/ptr"
 	"sigs.k8s.io/controller-runtime/pkg/client"
 	"sigs.k8s.io/controller-runtime/pkg/client/fake"
 	"sigs.k8s.io/controller-runtime/pkg/event"
 
 	configv1alpha1 "github.com/karmada-io/karmada/pkg/apis/config/v1alpha1"
+	policyv1alpha1 "github.com/karmada-io/karmada/pkg/apis/policy/v1alpha1"
 	workv1alpha2 "github.com/karmada-io/karmada/pkg/apis/work/v1alpha2"
 	"github.com/karmada-io/karmada/pkg/util"
 	"github.com/karmada-io/karmada/pkg/util/fedinformer/genericmanager"
@@ -2223,6 +2225,12 @@ func Test_createOrUpdateAttachedBinding(t *testing.T) {
 					Namespace:       "test",
 					ResourceVersion: "1000",
 					Labels:          map[string]string{"app": "nginx"},
+					OwnerReferences: []metav1.OwnerReference{
+						{
+							UID:        "foo-bar",
+							Controller: ptr.To[bool](true),
+						},
+					},
 				},
 				Spec: workv1alpha2.ResourceBindingSpec{
 					Resource: workv1alpha2.ObjectReference{
@@ -2273,6 +2281,12 @@ func Test_createOrUpdateAttachedBinding(t *testing.T) {
 					Namespace:       "test",
 					ResourceVersion: "1001",
 					Labels:          map[string]string{"app": "nginx", "foo": "bar"},
+					OwnerReferences: []metav1.OwnerReference{
+						{
+							UID:        "foo-bar",
+							Controller: ptr.To[bool](true),
+						},
+					},
 				},
 				Spec: workv1alpha2.ResourceBindingSpec{
 					Resource: workv1alpha2.ObjectReference{
@@ -2343,6 +2357,12 @@ func Test_createOrUpdateAttachedBinding(t *testing.T) {
 						Namespace:       "test",
 						ResourceVersion: "1000",
 						Labels:          map[string]string{"foo": "bar"},
+						OwnerReferences: []metav1.OwnerReference{
+							{
+								UID:        "foo-bar",
+								Controller: ptr.To[bool](true),
+							},
+						},
 					},
 					Spec: workv1alpha2.ResourceBindingSpec{
 						RequiredBy: []workv1alpha2.BindingSnapshot{
@@ -2389,6 +2409,12 @@ func Test_createOrUpdateAttachedBinding(t *testing.T) {
 					Name:      "test-binding",
 					Namespace: "test",
 					Labels:    map[string]string{"app": "nginx"},
+					OwnerReferences: []metav1.OwnerReference{
+						{
+							UID:        "foo-bar",
+							Controller: ptr.To[bool](true),
+						},
+					},
 				},
 				Spec: workv1alpha2.ResourceBindingSpec{
 					Resource: workv1alpha2.ObjectReference{
@@ -2439,6 +2465,12 @@ func Test_createOrUpdateAttachedBinding(t *testing.T) {
 					Namespace:       "test",
 					ResourceVersion: "1",
 					Labels:          map[string]string{"app": "nginx"},
+					OwnerReferences: []metav1.OwnerReference{
+						{
+							UID:        "foo-bar",
+							Controller: ptr.To[bool](true),
+						},
+					},
 				},
 				Spec: workv1alpha2.ResourceBindingSpec{
 					Resource: workv1alpha2.ObjectReference{
@@ -2486,6 +2518,300 @@ func Test_createOrUpdateAttachedBinding(t *testing.T) {
 				return fake.NewClientBuilder().WithScheme(Scheme).Build()
 			},
 		},
+		{
+			name: "update attached binding with ConflictResolution",
+			attachedBinding: &workv1alpha2.ResourceBinding{
+				ObjectMeta: metav1.ObjectMeta{
+					Name:            "test-binding",
+					Namespace:       "test",
+					ResourceVersion: "1000",
+					Labels:          map[string]string{"app": "nginx"},
+					OwnerReferences: []metav1.OwnerReference{
+						{
+							UID:        "foo-bar",
+							Controller: ptr.To[bool](true),
+						},
+					},
+				},
+				Spec: workv1alpha2.ResourceBindingSpec{
+					Resource: workv1alpha2.ObjectReference{
+						APIVersion:      "apps/v1",
+						Kind:            "Deployment",
+						Namespace:       "fake-ns",
+						Name:            "demo-app",
+						ResourceVersion: "22222",
+					},
+					RequiredBy: []workv1alpha2.BindingSnapshot{
+						{
+							Namespace: "test-1",
+							Name:      "test-binding-1",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "foo",
+									Replicas: 1,
+								},
+							},
+						},
+						{
+							Namespace: "default-2",
+							Name:      "default-binding-2",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "member2",
+									Replicas: 4,
+								},
+							},
+						},
+						{
+							Namespace: "test-2",
+							Name:      "test-binding-2",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "bar",
+									Replicas: 1,
+								},
+							},
+						},
+					},
+					ConflictResolution: policyv1alpha1.ConflictOverwrite,
+				},
+			},
+			wantErr: false,
+			wantBinding: &workv1alpha2.ResourceBinding{
+				ObjectMeta: metav1.ObjectMeta{
+					Name:            "test-binding",
+					Namespace:       "test",
+					ResourceVersion: "1001",
+					Labels:          map[string]string{"app": "nginx", "foo": "bar"},
+					OwnerReferences: []metav1.OwnerReference{
+						{
+							UID:        "foo-bar",
+							Controller: ptr.To[bool](true),
+						},
+					},
+				},
+				Spec: workv1alpha2.ResourceBindingSpec{
+					Resource: workv1alpha2.ObjectReference{
+						APIVersion:      "apps/v1",
+						Kind:            "Deployment",
+						Namespace:       "fake-ns",
+						Name:            "demo-app",
+						ResourceVersion: "22222",
+					},
+					RequiredBy: []workv1alpha2.BindingSnapshot{
+						{
+							Namespace: "default-1",
+							Name:      "default-binding-1",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "member1",
+									Replicas: 2,
+								},
+							},
+						},
+						{
+							Namespace: "default-2",
+							Name:      "default-binding-2",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "member2",
+									Replicas: 4,
+								},
+							},
+						},
+						{
+							Namespace: "default-3",
+							Name:      "default-binding-3",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "member3",
+									Replicas: 4,
+								},
+							},
+						},
+						{
+							Namespace: "test-1",
+							Name:      "test-binding-1",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "foo",
+									Replicas: 1,
+								},
+							},
+						},
+						{
+							Namespace: "test-2",
+							Name:      "test-binding-2",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "bar",
+									Replicas: 1,
+								},
+							},
+						},
+					},
+					ConflictResolution: policyv1alpha1.ConflictOverwrite,
+				},
+			},
+			setupClient: func() client.Client {
+				rb := &workv1alpha2.ResourceBinding{
+					ObjectMeta: metav1.ObjectMeta{
+						Name:            "test-binding",
+						Namespace:       "test",
+						ResourceVersion: "1000",
+						Labels:          map[string]string{"foo": "bar"},
+						OwnerReferences: []metav1.OwnerReference{
+							{
+								UID:        "foo-bar",
+								Controller: ptr.To[bool](true),
+							},
+						},
+					},
+					Spec: workv1alpha2.ResourceBindingSpec{
+						RequiredBy: []workv1alpha2.BindingSnapshot{
+							{
+								Namespace: "default-1",
+								Name:      "default-binding-1",
+								Clusters: []workv1alpha2.TargetCluster{
+									{
+										Name:     "member1",
+										Replicas: 2,
+									},
+								},
+							},
+							{
+								Namespace: "default-2",
+								Name:      "default-binding-2",
+								Clusters: []workv1alpha2.TargetCluster{
+									{
+										Name:     "member2",
+										Replicas: 3,
+									},
+								},
+							},
+							{
+								Namespace: "default-3",
+								Name:      "default-binding-3",
+								Clusters: []workv1alpha2.TargetCluster{
+									{
+										Name:     "member3",
+										Replicas: 4,
+									},
+								},
+							},
+						},
+					},
+				}
+				return fake.NewClientBuilder().WithScheme(Scheme).WithObjects(rb).Build()
+			},
+		},
+		{
+			name: "update attached binding which is being deleted",
+			attachedBinding: &workv1alpha2.ResourceBinding{
+				ObjectMeta: metav1.ObjectMeta{
+					Name:            "test-binding",
+					Namespace:       "test",
+					ResourceVersion: "1000",
+					Labels:          map[string]string{"app": "nginx"},
+					OwnerReferences: []metav1.OwnerReference{
+						{
+							UID:        "foo-bar",
+							Controller: ptr.To[bool](true),
+						},
+					},
+				},
+				Spec: workv1alpha2.ResourceBindingSpec{
+					Resource: workv1alpha2.ObjectReference{
+						APIVersion:      "apps/v1",
+						Kind:            "Deployment",
+						Namespace:       "fake-ns",
+						Name:            "demo-app",
+						ResourceVersion: "22222",
+					},
+					RequiredBy: []workv1alpha2.BindingSnapshot{
+						{
+							Namespace: "test-1",
+							Name:      "test-binding-1",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "foo",
+									Replicas: 1,
+								},
+							},
+						},
+					},
+					ConflictResolution: policyv1alpha1.ConflictOverwrite,
+				},
+			},
+			wantErr: true,
+			wantBinding: &workv1alpha2.ResourceBinding{
+				ObjectMeta: metav1.ObjectMeta{
+					Name:            "test-binding",
+					Namespace:       "test",
+					ResourceVersion: "1001",
+					Labels:          map[string]string{"app": "nginx", "foo": "bar"},
+					OwnerReferences: []metav1.OwnerReference{
+						{
+							UID:        "foo-bar",
+							Controller: ptr.To[bool](true),
+						},
+					},
+				},
+				Spec: workv1alpha2.ResourceBindingSpec{
+					Resource: workv1alpha2.ObjectReference{
+						APIVersion:      "apps/v1",
+						Kind:            "Deployment",
+						Namespace:       "fake-ns",
+						Name:            "demo-app",
+						ResourceVersion: "22222",
+					},
+					RequiredBy: []workv1alpha2.BindingSnapshot{
+						{
+							Namespace: "default-1",
+							Name:      "default-binding-1",
+							Clusters: []workv1alpha2.TargetCluster{
+								{
+									Name:     "member1",
+									Replicas: 2,
+								},
+							},
+						},
+					},
+					ConflictResolution: policyv1alpha1.ConflictOverwrite,
+				},
+			},
+			setupClient: func() client.Client {
+				rb := &workv1alpha2.ResourceBinding{
+					ObjectMeta: metav1.ObjectMeta{
+						Name:            "test-binding",
+						Namespace:       "test",
+						ResourceVersion: "1000",
+						Labels:          map[string]string{"foo": "bar"},
+						OwnerReferences: []metav1.OwnerReference{
+							{
+								UID:        "bar-foo",
+								Controller: ptr.To[bool](true),
+							},
+						},
+					},
+					Spec: workv1alpha2.ResourceBindingSpec{
+						RequiredBy: []workv1alpha2.BindingSnapshot{
+							{
+								Namespace: "default-1",
+								Name:      "default-binding-1",
+								Clusters: []workv1alpha2.TargetCluster{
+									{
+										Name:     "member1",
+										Replicas: 2,
+									},
+								},
+							},
+						},
+					},
+				}
+				return fake.NewClientBuilder().WithScheme(Scheme).WithObjects(rb).Build()
+			},
+		},
 	}
 	for _, tt := range tests {
 		t.Run(tt.name, func(t *testing.T) {
@@ -2496,10 +2822,13 @@ func Test_createOrUpdateAttachedBinding(t *testing.T) {
 			if (err != nil) != tt.wantErr {
 				t.Errorf("createOrUpdateAttachedBinding() error = %v, wantErr %v", err, tt.wantErr)
 			}
+			if tt.wantErr {
+				return
+			}
 			existBinding := &workv1alpha2.ResourceBinding{}
 			bindingKey := client.ObjectKeyFromObject(tt.attachedBinding)
 			err = d.Client.Get(context.TODO(), bindingKey, existBinding)
-			if (err != nil) != tt.wantErr {
+			if err != nil {
 				t.Errorf("createOrUpdateAttachedBinding(), Client.Get() error = %v, wantErr %v", err, tt.wantErr)
 			}
 			if !reflect.DeepEqual(existBinding, tt.wantBinding) {