diff --git a/packages/nextjs/src/config/webpack.ts b/packages/nextjs/src/config/webpack.ts
index cfc866cd36da..387d3344b8e8 100644
--- a/packages/nextjs/src/config/webpack.ts
+++ b/packages/nextjs/src/config/webpack.ts
@@ -79,7 +79,7 @@ export function constructWebpackConfigFunction(
         ],
       };
 
-      if (userSentryOptions.autoInstrumentServerFunctions) {
+      if (userSentryOptions.autoInstrumentServerFunctions !== false) {
         const pagesDir = newConfig.resolve?.alias?.['private-next-pages'] as string;
 
         // Default page extensions per https://github.com/vercel/next.js/blob/f1dbc9260d48c7995f6c52f8fbcc65f08e627992/packages/next/server/config-shared.ts#L161
diff --git a/packages/nextjs/test/integration/next.config.js b/packages/nextjs/test/integration/next.config.js
index a194a9b2bd3d..8992ed63d0e5 100644
--- a/packages/nextjs/test/integration/next.config.js
+++ b/packages/nextjs/test/integration/next.config.js
@@ -6,7 +6,6 @@ const moduleExports = {
   },
   pageExtensions: ['jsx', 'js', 'tsx', 'ts', 'page.tsx'],
   sentry: {
-    autoInstrumentServerFunctions: true,
     // Suppress the warning message from `handleSourcemapHidingOptionWarning` in `src/config/webpack.ts`
     // TODO (v8): This can come out in v8, because this option will get a default value
     hideSourceMaps: false,
diff --git a/packages/nextjs/test/integration/next10.config.template b/packages/nextjs/test/integration/next10.config.template
index 78d648416c1f..31c332cd25cd 100644
--- a/packages/nextjs/test/integration/next10.config.template
+++ b/packages/nextjs/test/integration/next10.config.template
@@ -7,7 +7,6 @@ const moduleExports = {
   },
   pageExtensions: ['jsx', 'js', 'tsx', 'ts', 'page.tsx'],
   sentry: {
-    autoInstrumentServerFunctions: true,
     // Suppress the warning message from `handleSourcemapHidingOptionWarning` in `src/config/webpack.ts`
     // TODO (v8): This can come out in v8, because this option will get a default value
     hideSourceMaps: false,
diff --git a/packages/nextjs/test/integration/next11.config.template b/packages/nextjs/test/integration/next11.config.template
index bd177f0d3e82..6a7e849067b1 100644
--- a/packages/nextjs/test/integration/next11.config.template
+++ b/packages/nextjs/test/integration/next11.config.template
@@ -8,7 +8,6 @@ const moduleExports = {
   },
   pageExtensions: ['jsx', 'js', 'tsx', 'ts', 'page.tsx'],
   sentry: {
-    autoInstrumentServerFunctions: true,
     // Suppress the warning message from `handleSourcemapHidingOptionWarning` in `src/config/webpack.ts`
     // TODO (v8): This can come out in v8, because this option will get a default value
     hideSourceMaps: false,