diff --git a/eng/pipelines/coreclr/templates/helix-queues-setup.yml b/eng/pipelines/coreclr/templates/helix-queues-setup.yml
index ae1b15da67a740..bd12761533e8a3 100644
--- a/eng/pipelines/coreclr/templates/helix-queues-setup.yml
+++ b/eng/pipelines/coreclr/templates/helix-queues-setup.yml
@@ -91,14 +91,12 @@ jobs:
       - ${{ if and(eq(variables['System.TeamProject'], 'public'), notIn(parameters.jobParameters.helixQueueGroup, 'pr', 'ci', 'libraries')) }}:
         - (Debian.10.Amd64.Open)Ubuntu.1804.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:debian-10-helix-amd64-20210304164434-56c6673
         - (Debian.11.Amd64.Open)Ubuntu.1804.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:debian-11-helix-amd64-20210304164428-5a7c380
-        - Ubuntu.1604.Amd64.Open
         - Ubuntu.1804.Amd64.Open
         - (Centos.8.Amd64.Open)Ubuntu.1604.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:centos-8-helix-20201229003624-c1bf759
         - RedHat.7.Amd64.Open
       - ${{ if eq(variables['System.TeamProject'], 'internal') }}:
         - (Debian.10.Amd64)Ubuntu.1804.amd64@mcr.microsoft.com/dotnet-buildtools/prereqs:debian-10-helix-amd64-20210304164434-56c6673
         - (Debian.11.Amd64)Ubuntu.1804.amd64@mcr.microsoft.com/dotnet-buildtools/prereqs:debian-11-helix-amd64-20210304164428-5a7c380
-        - Ubuntu.1604.Amd64
         - Ubuntu.1804.Amd64
         - (Centos.8.Amd64)Ubuntu.1604.amd64@mcr.microsoft.com/dotnet-buildtools/prereqs:centos-8-helix-20201229003624-c1bf759
         - (Fedora.34.Amd64)Ubuntu.1604.amd64@mcr.microsoft.com/dotnet-buildtools/prereqs:fedora-34-helix-20210728124700-4f64125
diff --git a/eng/pipelines/libraries/helix-queues-setup.yml b/eng/pipelines/libraries/helix-queues-setup.yml
index 80d23e19ae3228..8c7cd7d8fc1959 100644
--- a/eng/pipelines/libraries/helix-queues-setup.yml
+++ b/eng/pipelines/libraries/helix-queues-setup.yml
@@ -65,7 +65,6 @@ jobs:
           - ${{ if eq(parameters.jobParameters.isFullMatrix, true) }}:
             - (Centos.8.Amd64.Open)Ubuntu.1604.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:centos-8-helix-20201229003624-c1bf759
             - RedHat.7.Amd64.Open
-            - Ubuntu.1604.Amd64.Open
             - Ubuntu.1804.Amd64.Open
             - SLES.12.Amd64.Open
             - SLES.15.Amd64.Open
@@ -79,7 +78,6 @@ jobs:
             - RedHat.7.Amd64.Open
             - (Debian.10.Amd64.Open)Ubuntu.1804.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:debian-10-helix-amd64-20210304164434-56c6673
             - (Debian.11.Amd64.Open)Ubuntu.1804.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:debian-11-helix-amd64-20210304164428-5a7c380
-            - Ubuntu.1604.Amd64.Open
             - Ubuntu.1804.Amd64.Open
             - SLES.15.Amd64.Open
             - (Fedora.34.Amd64.Open)ubuntu.1604.amd64.open@mcr.microsoft.com/dotnet-buildtools/prereqs:fedora-34-helix-20210728124700-4f64125