diff --git a/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.targets b/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.targets
index 2e134e663a493b..696fa19c03afc8 100755
--- a/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.targets
+++ b/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.targets
@@ -214,7 +214,6 @@ The .NET Foundation licenses this file to you under the MIT license.
       <IlcArg Include="@(MibcFile->'--mibc:%(Identity)')" />
       <IlcArg Condition="$(IlcGenerateMetadataLog) == 'true'" Include="--metadatalog:$(NativeIntermediateOutputPath)%(ManagedBinary.Filename).metadata.csv" />
       <IlcArg Condition="$(TargetArchitecture) != ''" Include="--targetarch:$(TargetArchitecture)" />
-      <IlcArg Condition="$(TargetOS) != ''" Include="--targetos:$(TargetOS)" />
       <IlcArg Condition="$(IlcMultiModule) == 'true'" Include="--multifile" />
       <IlcArg Condition="$(IlcMultiModule) != 'true' and '$(IlcDehydrate)' != 'false' and '$(ControlFlowGuard)' != 'Guard'" Include="--dehydrate" />
       <IlcArg Condition="$(Optimize) == 'true'" Include="-O" />