diff --git a/Cargo.Bazel.lock b/Cargo.Bazel.lock
index 282508b5c..f61606d84 100644
--- a/Cargo.Bazel.lock
+++ b/Cargo.Bazel.lock
@@ -1,5 +1,5 @@
 {
-  "checksum": "86820685790dc151c444b969cb6cb2ce2835933116a79bd69d9979b0df78d0db",
+  "checksum": "2bda21c3422f1e2a634bfe6e3d48e0041bd5dbb73f9d63b191e5393c2e338c19",
   "crates": {
     "actix-codec 0.5.2": {
       "name": "actix-codec",
@@ -28157,23 +28157,29 @@
         ],
         "crate_features": {
           "common": [
-            "elf",
-            "errno",
             "general",
             "ioctl",
             "no_std"
           ],
           "selects": {
             "aarch64-unknown-linux-gnu": [
+              "elf",
+              "errno",
               "std"
             ],
             "arm-unknown-linux-gnueabi": [
+              "elf",
+              "errno",
               "std"
             ],
             "armv7-unknown-linux-gnueabi": [
+              "elf",
+              "errno",
               "std"
             ],
             "i686-unknown-linux-gnu": [
+              "elf",
+              "errno",
               "std"
             ],
             "powerpc-unknown-linux-gnu": [
@@ -28183,6 +28189,8 @@
               "std"
             ],
             "x86_64-unknown-linux-gnu": [
+              "elf",
+              "errno",
               "std"
             ]
           }
@@ -29397,6 +29405,10 @@
               "id": "regex 1.10.3",
               "target": "regex"
             },
+            {
+              "id": "retry 2.0.0",
+              "target": "retry"
+            },
             {
               "id": "serde 1.0.196",
               "target": "serde"
diff --git a/Cargo.lock b/Cargo.lock
index 6810b66bb..79b38f594 100644
--- a/Cargo.lock
+++ b/Cargo.lock
@@ -5909,6 +5909,7 @@ dependencies = [
  "multiservice-discovery-shared",
  "regex",
  "reqwest",
+ "retry",
  "serde",
  "serde_json",
  "service-discovery",
diff --git a/rs/ic-observability/multiservice-discovery/Cargo.toml b/rs/ic-observability/multiservice-discovery/Cargo.toml
index 132ee4506..85b65433a 100644
--- a/rs/ic-observability/multiservice-discovery/Cargo.toml
+++ b/rs/ic-observability/multiservice-discovery/Cargo.toml
@@ -31,6 +31,7 @@ tokio = { workspace = true }
 url = { workspace = true }
 futures.workspace = true
 axum = "0.7.4"
+retry = { workspace = true }
 
 [dev-dependencies]
 tempfile = { workspace = true }
diff --git a/rs/ic-observability/multiservice-discovery/src/definition.rs b/rs/ic-observability/multiservice-discovery/src/definition.rs
index c10199bed..23deb9285 100644
--- a/rs/ic-observability/multiservice-discovery/src/definition.rs
+++ b/rs/ic-observability/multiservice-discovery/src/definition.rs
@@ -3,6 +3,8 @@ use crossbeam_channel::Sender;
 use futures_util::future::join_all;
 use ic_management_types::Network;
 use ic_registry_client::client::ThresholdSigPublicKey;
+use serde::Deserialize;
+use serde::Serialize;
 use service_discovery::job_types::JobType;
 use service_discovery::registry_sync::Interrupted;
 use service_discovery::IcServiceDiscovery;
@@ -16,6 +18,8 @@ use std::collections::HashSet;
 use std::error::Error;
 use std::fmt::Debug;
 use std::fmt::{Display, Error as FmtError, Formatter};
+use std::fs;
+use std::io::Write;
 use std::net::SocketAddr;
 use std::sync::Arc;
 use std::{
@@ -25,6 +29,33 @@ use std::{
 use tokio::sync::Mutex;
 use url::Url;
 
+use crate::make_logger;
+
+#[derive(Clone, Serialize, Deserialize)]
+pub struct FSDefinition {
+    pub nns_urls: Vec<Url>,
+    pub registry_path: PathBuf,
+    pub name: String,
+    pub public_key: Option<ThresholdSigPublicKey>,
+    pub poll_interval: Duration,
+    pub registry_query_timeout: Duration,
+    pub boundary_nodes: Vec<BoundaryNode>,
+}
+
+impl From<Definition> for FSDefinition {
+    fn from(definition: Definition) -> Self {
+        Self {
+            nns_urls: definition.nns_urls, 
+            registry_path: definition.registry_path,
+            name: definition.name,
+            public_key: definition.public_key,
+            poll_interval: definition.poll_interval,
+            registry_query_timeout: definition.registry_query_timeout,
+            boundary_nodes: definition.boundary_nodes
+        }
+    }
+}
+
 #[derive(Clone)]
 pub struct Definition {
     pub nns_urls: Vec<Url>,
@@ -38,6 +69,20 @@ pub struct Definition {
     pub boundary_nodes: Vec<BoundaryNode>,
 }
 
+impl From<FSDefinition> for Definition {
+    fn from(fs_definition: FSDefinition) -> Self {
+        Definition::new(
+            fs_definition.nns_urls, 
+            fs_definition.registry_path, 
+            fs_definition.name, 
+            make_logger(), 
+            fs_definition.public_key, 
+            fs_definition.poll_interval, 
+            fs_definition.registry_query_timeout
+        )
+    }
+}
+
 impl Debug for Definition {
     fn fmt(&self, f: &mut Formatter<'_>) -> Result<(), FmtError> {
         write!(
@@ -300,7 +345,7 @@ impl RunningDefinition {
     }
 }
 
-#[derive(Clone)]
+#[derive(Clone, Serialize, Deserialize)]
 pub struct BoundaryNode {
     pub name: String,
     pub targets: BTreeSet<SocketAddr>,
@@ -394,6 +439,42 @@ impl DefinitionsSupervisor {
         }
     }
 
+    pub(crate) async fn load_or_create_defs(&self, networks_state_file: PathBuf) -> Result<(), Box<dyn Error>> {
+        if networks_state_file.exists() {
+            let file_content = fs::read_to_string(networks_state_file)?;
+            let initial_definitions: Vec<FSDefinition> = serde_json::from_str(&file_content)?;
+            self.start(
+                initial_definitions.into_iter().map(|def| def.into()).collect(),
+                StartMode::AddToDefinitions,
+            )
+            .await?;
+        }
+        Ok(())
+    }
+
+    pub(crate) async fn persist_defs(&self, networks_state_file: PathBuf) -> Result<(), Box<dyn Error>> {
+        let existing = self.definitions.lock().await;
+        retry::retry(retry::delay::Exponential::from_millis(10).take(5), || {
+            std::fs::OpenOptions::new()
+                .create(true)
+                .write(true)
+                .open(&networks_state_file.as_path())
+                .and_then(|mut file| {
+                    let fs_def: Vec<FSDefinition> = existing
+                    .values()
+                    .cloned()
+                    .into_iter()
+                    .map(|running_def| running_def.definition.into())
+                    .collect::<Vec<_>>(); 
+
+                    file.write_all(serde_json::to_string(&fs_def)?.as_bytes())
+                        .map(|_| file)
+                })
+                .and_then(|mut file| file.flush())
+        })?;
+        Ok(())
+    }
+
     async fn start_inner(
         &self,
         existing: &mut BTreeMap<String, RunningDefinition>,
diff --git a/rs/ic-observability/multiservice-discovery/src/main.rs b/rs/ic-observability/multiservice-discovery/src/main.rs
index a9178df81..5fe3110d7 100644
--- a/rs/ic-observability/multiservice-discovery/src/main.rs
+++ b/rs/ic-observability/multiservice-discovery/src/main.rs
@@ -67,6 +67,10 @@ fn main() {
         }
     } else {
         let supervisor = DefinitionsSupervisor::new(rt.handle().clone(), cli_args.start_without_mainnet);
+        if let Some(networks_state_file) = cli_args.networks_state_file.clone() {
+            rt.block_on(supervisor.load_or_create_defs(networks_state_file)).unwrap();
+        }
+
         let (server_stop, server_stop_receiver) = oneshot::channel();
 
         //Configure server
@@ -98,6 +102,11 @@ fn main() {
         // Signal server to stop.  Stop happens in parallel with supervisor stop.
         server_stop.send(()).unwrap();
 
+        // Persist definitions to disk before ending the supervisor.
+        if let Some(networks_state_file) = cli_args.networks_state_file.clone() {
+            rt.block_on(supervisor.persist_defs(networks_state_file)).unwrap();
+        }
+        
         //Stop all definitions.  End happens in parallel with server stop.
         rt.block_on(supervisor.end());
 
@@ -180,4 +189,15 @@ the Prometheus targets of mainnet as a JSON structure on stdout.
 "#
     )]
     render_prom_targets_to_stdout: bool,
+    
+    #[clap(
+        long = "networks-state-file",
+        default_value = None,
+        action,
+        help = r#"
+Preload networks definitions from file path. In case the file does not
+exist, it will be created.
+"#
+    )]
+    networks_state_file: Option<PathBuf>,
 }