diff --git a/dandi/consts.py b/dandi/consts.py
index b68dff98f..a3506ceb7 100644
--- a/dandi/consts.py
+++ b/dandi/consts.py
@@ -148,7 +148,7 @@ class DandiInstance(NamedTuple):
 #: of retries)
 RETRY_STATUSES = (500, 502, 503, 504)
 
-VIDEO_FILE_EXTENSIONS = [".mp4", ".avi", ".wmv", ".mov", ".flv"]
+VIDEO_FILE_EXTENSIONS = [".mp4", ".avi", ".wmv", ".mov", ".flv", ".mkv"]
 VIDEO_FILE_MODULES = ["processing", "acquisition"]
 
 #: Maximum allowed depth of a Zarr directory tree