diff --git a/crates/ruff_python_parser/src/lexer.rs b/crates/ruff_python_parser/src/lexer.rs
index c2aa3772898fe9..3ba20e78e88e87 100644
--- a/crates/ruff_python_parser/src/lexer.rs
+++ b/crates/ruff_python_parser/src/lexer.rs
@@ -620,7 +620,7 @@ impl<'source> Lexer<'source> {
                     }
                 }
                 '{' => {
-                    if self.cursor.second() == '{' {
+                    if self.cursor.second() == '{' && !fstring.is_in_format_spec(self.nesting) {
                         self.cursor.bump();
                         normalized
                             .push_str(&self.source[TextRange::new(last_offset, self.offset())]);
@@ -2047,7 +2047,7 @@ def f(arg=%timeit a = b):
 
     #[test]
     fn test_fstring_with_format_spec() {
-        let source = r#"f"{foo:} {x=!s:.3f} {x:.{y}f} {'':*^{1:{1}}}""#;
+        let source = r#"f"{foo:} {x=!s:.3f} {x:.{y}f} {'':*^{1:{1}}} {x:{{1}.pop()}}""#;
         assert_debug_snapshot!(lex_source(source));
     }
 
diff --git a/crates/ruff_python_parser/src/snapshots/ruff_python_parser__lexer__tests__fstring_with_format_spec.snap b/crates/ruff_python_parser/src/snapshots/ruff_python_parser__lexer__tests__fstring_with_format_spec.snap
index c41165118ff93f..db324bdef0c2dd 100644
--- a/crates/ruff_python_parser/src/snapshots/ruff_python_parser__lexer__tests__fstring_with_format_spec.snap
+++ b/crates/ruff_python_parser/src/snapshots/ruff_python_parser__lexer__tests__fstring_with_format_spec.snap
@@ -191,11 +191,76 @@ expression: lex_source(source)
         43..44,
     ),
     (
-        FStringEnd,
+        FStringMiddle {
+            value: " ",
+            is_raw: false,
+        },
         44..45,
     ),
+    (
+        Lbrace,
+        45..46,
+    ),
+    (
+        Name {
+            name: "x",
+        },
+        46..47,
+    ),
+    (
+        Colon,
+        47..48,
+    ),
+    (
+        Lbrace,
+        48..49,
+    ),
+    (
+        Lbrace,
+        49..50,
+    ),
+    (
+        Int {
+            value: 1,
+        },
+        50..51,
+    ),
+    (
+        Rbrace,
+        51..52,
+    ),
+    (
+        Dot,
+        52..53,
+    ),
+    (
+        Name {
+            name: "pop",
+        },
+        53..56,
+    ),
+    (
+        Lpar,
+        56..57,
+    ),
+    (
+        Rpar,
+        57..58,
+    ),
+    (
+        Rbrace,
+        58..59,
+    ),
+    (
+        Rbrace,
+        59..60,
+    ),
+    (
+        FStringEnd,
+        60..61,
+    ),
     (
         Newline,
-        45..45,
+        61..61,
     ),
 ]