diff --git a/src/python/grpcio_tests/tests/observability/BUILD.bazel b/src/python/grpcio_tests/tests/observability/BUILD.bazel
index 29bd6a90e6090..465fb7c2b8dc9 100644
--- a/src/python/grpcio_tests/tests/observability/BUILD.bazel
+++ b/src/python/grpcio_tests/tests/observability/BUILD.bazel
@@ -18,6 +18,11 @@ py_library(
     srcs = ["_test_server.py"],
 )
 
+py_library(
+    name = "_from_observability_import_star",
+    srcs = ["_from_observability_import_star.py"],
+)
+
 py_test(
     name = "_observability_test",
     size = "small",
@@ -45,3 +50,16 @@ py_test(
         "//src/python/grpcio_tests/tests/testing",
     ],
 )
+
+py_test(
+    name = "_observability_api_test",
+    size = "small",
+    srcs = ["_observability_api_test.py"],
+    imports = ["../../"],
+    main = "_observability_api_test.py",
+    deps = [
+        ":_from_observability_import_star",
+        "//src/python/grpcio_observability/grpc_observability:pyobservability",
+        "//src/python/grpcio_tests/tests/testing",
+    ],
+)
diff --git a/src/python/grpcio_tests/tests/observability/_from_observability_import_star.py b/src/python/grpcio_tests/tests/observability/_from_observability_import_star.py
new file mode 100644
index 0000000000000..e218c49cdb231
--- /dev/null
+++ b/src/python/grpcio_tests/tests/observability/_from_observability_import_star.py
@@ -0,0 +1,25 @@
+# Copyright 2024 gRPC authors.
+#
+# Licensed under the Apache License, Version 2.0 (the "License");
+# you may not use this file except in compliance with the License.
+# You may obtain a copy of the License at
+#
+#     http://www.apache.org/licenses/LICENSE-2.0
+#
+# Unless required by applicable law or agreed to in writing, software
+# distributed under the License is distributed on an "AS IS" BASIS,
+# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+# See the License for the specific language governing permissions and
+# limitations under the License.
+
+_BEFORE_IMPORT = tuple(globals())
+
+from grpc_observability import *  # pylint: disable=wildcard-import,unused-wildcard-import
+
+_AFTER_IMPORT = tuple(globals())
+
+GRPC_OBSERVABILITY_ELEMENTS = tuple(
+    element
+    for element in _AFTER_IMPORT
+    if element not in _BEFORE_IMPORT and element != "_BEFORE_IMPORT"
+)
diff --git a/src/python/grpcio_tests/tests/observability/_observability_api_test.py b/src/python/grpcio_tests/tests/observability/_observability_api_test.py
new file mode 100644
index 0000000000000..3d5fb093d6b4e
--- /dev/null
+++ b/src/python/grpcio_tests/tests/observability/_observability_api_test.py
@@ -0,0 +1,39 @@
+# Copyright 2024 gRPC authors.
+#
+# Licensed under the Apache License, Version 2.0 (the "License");
+# you may not use this file except in compliance with the License.
+# You may obtain a copy of the License at
+#
+#     http://www.apache.org/licenses/LICENSE-2.0
+#
+# Unless required by applicable law or agreed to in writing, software
+# distributed under the License is distributed on an "AS IS" BASIS,
+# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+# See the License for the specific language governing permissions and
+# limitations under the License.
+"""Test of gRPC Python Observability's application-layer API."""
+
+import logging
+import unittest
+
+import grpc_observability
+
+from tests.observability import _from_observability_import_star
+
+
+class AllTest(unittest.TestCase):
+    def testAll(self):
+        expected_observability_code_elements = (
+            "OpenTelemetryObservability",
+            "OpenTelemetryPlugin",
+        )
+
+        self.assertCountEqual(
+            expected_observability_code_elements,
+            _from_observability_import_star.GRPC_OBSERVABILITY_ELEMENTS,
+        )
+
+
+if __name__ == "__main__":
+    logging.basicConfig()
+    unittest.main(verbosity=2)
diff --git a/src/python/grpcio_tests/tests/tests.json b/src/python/grpcio_tests/tests/tests.json
index 7ba494ac5b611..f0974c125db73 100644
--- a/src/python/grpcio_tests/tests/tests.json
+++ b/src/python/grpcio_tests/tests/tests.json
@@ -10,6 +10,7 @@
   "tests.interop._insecure_intraop_test.InsecureIntraopTest",
   "tests.interop._secure_intraop_test.SecureIntraopTest",
   "tests.observability._observability_test.ObservabilityTest",
+  "tests.observability._observability_api_test.AllTest",
   "tests.observability._open_telemetry_observability_test.OpenTelemetryObservabilityTest",
   "tests.protoc_plugin._python_plugin_test.ModuleMainTest",
   "tests.protoc_plugin._python_plugin_test.PythonPluginTest",