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2019 Philadelphia CCDL Training

Location: 1429 Walnut St. Floor 10 Philadelphia, PA 19102

Dates: 10/14/2019 - 10/16/2019

Time Topic
Day 1
9:00 AM Welcome
9:10 Getting Started: Install Docker, Get files from flash drives, Docker container set up
10:00 Break
10:15 Introduction to R
11:00 R Intermediate Topics (including Tidyverse)
11:45 Exercises Introduction: Intro to R, Intro to Tidyverse
12:30 Lunch
1:30 PM Bulk RNA-seq I: QC, trim, and quantification with Salmon
2:00 Bulk RNA-seq II: Gene-Level Summaries with tximport
2:45 Break
3:00 Bulk RNA-seq III: Exploratory Analyses
3:15 Bulk RNA-seq IV: Differential Expression Analysis: tximport, Differential Expression
4:45 Exercise Introduction: Bulk RNA-seq
5:00 End
Day 2
9:00 AM scRNA-seq I: Normalizing a count matrix
10:15 Break
10:30 scRNA-seq II: Processing 10X data from raw
12:30 PM Lunch
2:00 scRNA-seq III: Dimensionality reduction
2:45 Exercise Introduction: scRNA-seq
3:00 Break
3:30 Machine Learning: Data Preparation, Clustering, PLIER, Differential PLIER LVs
4:45 Exercise Introduction: Machine Learning
5:00 End
6:00 Dinner: Nomad Roman
Day 3
9:00 AM Analyze your own data (or some stuff we have)!
12:30 PM Lunch
2:00 Presentations, interpretations, and discussion
3:30 Break
4:00 Talk from CCDL data science team and wrap-up
5:00 Adjourn